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Recombinant Carboxypeptidase B in E.coli Carboxypeptidase B for mass spectrometry

Categories Recombinant Carboxypeptidase B
Brand Name: YaxinBio
Model Number: SRCPB01
Certification: ISO 9001
Place of Origin: China
MOQ: 100ug
Payment Terms: T/T
Packaging Details: ice packaging
Price: 198$/100ug
Supply Ability: 1000g per week
Product: Sequencing Grade Carboxypeptidase B
Manufacturer: YaxinBio
Source: E. Coli
Purified by: HPLC
Contaminant activity: No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant.
RELATED PRODUCT: Recombinant Chymotrypsin (sequencing grade);
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    Recombinant Carboxypeptidase B in E.coli Carboxypeptidase B for mass spectrometry

    Recombinant Carboxypeptidase B in E.coli Carboxypeptidase B for Mass Spectrometry


    Description


    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.


    Main Features


    Purity: Greater than 95% as determined by SDS-PAGE.

    Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.

    Bilogical Activity:170 units/mg protein.


    Application


    Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

    Antibody quality control.


    Recommend Usage


    Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


    Storage Instructions


    Stability:Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below under 2-8℃ in sealed container. For long term storage it is recommended to be stored under -20℃. Please prevent freeze-thaw cycles.


    Email:Zhaozhi@yaxinbio.com

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